ICSI (Intra Cytoplasmic Sperm Injection)

ICSI (Intra Cytoplasmic Sperm Injection) is the procedure in which a spermatozoon is injected directly into an egg cell with the help of a micro-needle. This method is very efficient and usually is used in the heavy forms of masculine infertility, when the quantitative or qualitative indexes of sperm are considerably reduced.       

With the help of micromanipulators, the best quality sperm is selected under a microscope. It is fixed in a micro-needle, by which the puncture of a shiny shell of the egg cell is carried out, and then the sperm is injected into the cytoplasm of the egg cell. Thus, the fertilization of all egg cells, obtained during the puncture, is carried out.

As a result of fertilization, on the next day a zygote is formed containing two nuclei – mother’s and father’s. From it the embryo will develop itself.

In our clinic, the ICSI procedure is performed by the latest and most up-to-date micro-manipulator The Integra Ti ™.

 Stages of ICSI:

  • The mature egg cell is held by a specialized pipette.
  • A very delicate, sharp and hollow needle is used to immobilize and pick up a single sperm of good quality.
  • Then this needle is inserted through the shell of the egg cell into the cytoplasm
  • The sperm is injected in the cytoplasm and the needle is removed.
  • The egg cells are checked the next morning for evidence of normal fertilization.

Indications for the ICSI method:

  oligozoospermia – the concentration of spermatozoa less than 2 million / ml;
  stenozoospermia – the number of active spermatozoa less than 1 million mobile sperm in 1 ml of ejaculate;
  teratozoospermia – less than 10% of spermatozoa of normal morphological structure;
  the presence of high titer of antisperm antibodies in ejaculate, which will interfere the natural fertilization, even in cases of normal concentration of sperm;
  absence of fertilization in previous IVF attempts;
  the age of a woman is more than 38 years, a man is more than 45 years old.

In general, the incidence of pregnancy after ICSI in our clinic is high, 45-50%.

If you faced with a problem of infertility treatment, the specialists of the Clinic of Professor Stefan Khmil will do all the best to help you.

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